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A novel dehydroabietic acid-based fluorescent probe for detection of Fe3+ and Hg2+ ions and its application in live-cell imaging.
- Source :
-
Microchemical Journal . Jan2021:Part B, Vol. 160, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- • Dehydroabietate-based fluorescent probe for Hg2+ and Fe3+ was synthesized and characterized. • Detection limits of the sensor were 31.0 nM and 31.2 nM for Hg2+ and Fe3+ ions, respectively. • The sensor have a wide pH range for ion detection (5 ~ 12), and a short response time (10 s). • Fluorescent imaging experiments using MCF-7 and SMCC-7721 cell were carried out successfully. A novel dehydroabietic acid-based fluorescent probe (DBH) containing 2,4-diarylbenzimidazole moiety was designed and synthesized, and the compound was characterized by its UV–Vis, FT-IR, 1H NMR, 13C NMR and ESI-MS spectra. DBH could be used for the detection of Fe3+ and Hg2+ through the selective fluorescence quenching in EtOH/H 2 O solution. The probe DBH showed excellent selectivity and sensitivity towards Fe3+ and Hg2+ over other metal ions. The limits of detection were calculated as 3.12 × 10−8 M and 3.10 × 10−8 M, respectively. Especially, DBH have a wide pH range for ion detection (5 ~ 12), and a short response time (10 s). Job's plot method in combination with ESI-MS and FT-IR spectra was used to determine the stoichiometry of DBH -Fe3+/Hg2+ complexes as both 2:1. Moreover, DBH exhibited low cytotoxicity to MCF-7 and SMCC-7721 cells (IC 50 > 100 μM). In the fluorescent cell imaging experiments, DBH could be used as a prominent intracellular probe for the detection of Fe3+ and Hg2+ in living cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CELL imaging
*IONS
*FLUORESCENCE quenching
*METAL ions
*DETECTION limit
Subjects
Details
- Language :
- English
- ISSN :
- 0026265X
- Volume :
- 160
- Database :
- Academic Search Index
- Journal :
- Microchemical Journal
- Publication Type :
- Academic Journal
- Accession number :
- 147460334
- Full Text :
- https://doi.org/10.1016/j.microc.2020.105682