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Polymer-functionalized carbon nanotubes prepared via ring-opening polymerization for electrochemical detection of carcinoembryonic antigen.

Authors :
Li, Jinge
Zhao, Liying
Wang, Wenbin
Liu, Yanju
Yang, Huaixia
Kong, Jinming
Si, Fuchun
Source :
Sensors & Actuators B: Chemical. Feb2021, Vol. 328, pN.PAG-N.PAG. 1p.
Publication Year :
2021

Abstract

• ROP is first time used for highly sensitive detection of CEA. • A detection limit of 0.28 fg mL−1 is achieved based on Apt2-CNTs-PFcGE composites. • The proposed sensor shows high sensitivity, selectivity, low cost, simplicity, and good biocompatibility. • This strategy may hold great potential in biomedical research and clinical diagnosis. Carcinoembryonic antigen (CEA) is regarded as the most common tumor biomarker, the precise and sensitive measurement of which has great significance for the cancer diagnosis, treatment, and prognosis. Herein, for the first time, we construct a novel sandwich-type electrochemical aptasensor for CEA detection based on polymer-functionalized carbon nanotubes (CNTs) composites prepared by ring-opening polymerization (ROP). CEA-aptamer 1 (Apt 1) is self-assembled on a gold electrode surface and used as a probe for CEA capture. CNTs are used as the substrates for polymer chain growth to prepare poly(ferrocenyl glycidyl ether)-grafted CNTs (PFcGE-CNTs) composites by ROP. Due to the efficient polymerization and good biocompatibility of ROP technology, PFcGE-CNTs composites can provide a hydrophilic-biocompatible nanoplatform for CEA-aptamer 2 (Apt 2) capture and serve as a signal amplifier. Remarkably, PFcGE-CNTs composites feature the characteristics of good conductivity and abundant ferrocene derivatives, which significantly amplify the electrochemical signal. Under optimized conditions, the fabricated aptasensor shows a wide detection range of 1 × 10−15–1 × 10−8 g mL−1 and a limit of detection (LOD) down to 2.8 × 10−16 g mL−1, which are superior to those of most reported electrochemical methods. Furthermore, the proposed aptasensor exhibits excellent applicability for CEA detection in practical sample analysis, which is of promising application potential for the early diagnosis and monitoring of cancer. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09254005
Volume :
328
Database :
Academic Search Index
Journal :
Sensors & Actuators B: Chemical
Publication Type :
Academic Journal
Accession number :
147504147
Full Text :
https://doi.org/10.1016/j.snb.2020.129031