Live-cell superresolution pathology reveals different molecular mechanisms of pelizaeus-merzbacher disease.

Modern cellular pathology relies on conventional microscopes to study the etiology and pathogenesis of diseases, which suffer from limited spatial resolution, little dynamic information due to sample fixation, and the lack of molecular information. Different PLP1 mutants exhibited distinct localizations; for example, while PLP1 C353G-EGFP exhibited patterns similar to WT PLP1 (Fig. Overall, we envision that live-cell SR pathology enabled by Hessian deconvolution confers a much-needed tool, which can be applied to PMD-like diseases and other rare diseases caused by dominant gene mutations. [Extracted from the article]