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Biohythane production and microbial characteristics of two alternating mesophilic and thermophilic two-stage anaerobic co-digesters fed with rice straw and pig manure.
- Source :
-
Bioresource Technology . Jan2021:Part A, Vol. 320, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- • Biohythane can be produced by anaerobic co-digestion of rice straw and pig manure. • The thermophilic-mesophilic systems possessed had high hydrogen and methane yields. • Hydrolysis was the rate-limiting step of the thermophilic acid-producing phase. • Methanobrevibacter dominated in mesophilic acid-producing phase with a low H 2 yield. • The thermophilic acid-producing phase provided selective pressure for methanogens. To investigate biohythane production and microbial behavior during temperature-phased (TP) anaerobic co-digestion (AcD) of rice straw (RS) and pig manure (PM), a mesophilic-thermophilic (M1-T1) AcD system and a thermophilic-mesophilic (T2-M2) AcD system were continuously operated for 95 days in parallel. The maximal ratio (8.44%v/v) of produced hydrogen to methane demonstrated the feasibility of biohythane production by co-digestion of RS and PM. T2-M2 exhibited higher hydrogen (16.68 ± 1.88 mL/gVS) and methane (197.73 ± 11.77 mL/gVS) yields than M1-T1 (3.08 ± 0.39 and 109.03 ± 4.97 mL/gVS, respectively). Methanobrevibacter (75.62%, a hydrogenotrophic methanogen) dominated in the M1 reactor, resulting in low hydrogen production. Hydrogen-producing bacteria (Thermoanaerobacterium 32.06% and Clostridium_sensu_stricto_1 27.33%) dominated in T2, but the abundance of hydrolytic bacteria was low, indicating that hydrolysis could be a rate-limiting step. The thermophilic acid-producing phase provided effective selective pressure for hydrogen-consuming microbes, and the high diversity of microbes in M2 implied a more efficient pathway of methane production. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09608524
- Volume :
- 320
- Database :
- Academic Search Index
- Journal :
- Bioresource Technology
- Publication Type :
- Academic Journal
- Accession number :
- 147506362
- Full Text :
- https://doi.org/10.1016/j.biortech.2020.124303