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Development of a gold nanoparticle-based strip assay for detection of clopidol in the chicken.
- Source :
-
Food & Agricultural Immunology . Dec2020, Vol. 31 Issue 1, p489-500. 12p. - Publication Year :
- 2020
-
Abstract
- In this study, a monoclonal antibody with selectivity for clopidol was produced based on a new hapten. The 50% inhibition concentration (IC50) for the developed ic-ELISA was 0.275 ng/mL. Cross-reactivity tests showed that the monoclonal antibody had high specificity for clopidol. Using this antibody, a lateral-flow colloidal gold immunoassay (LCGA) was created for the rapid screening of clopidol in raw chicken samples. With the naked eye, the cut-off value of the strip assay was 5 μg/kg for clopidol in chicken samples. Therefore, this LCGA provides a sensitive and rapid method for on-site detection of clopidol in chicken samples. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09540105
- Volume :
- 31
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Food & Agricultural Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 147528187
- Full Text :
- https://doi.org/10.1080/09540105.2020.1737655