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Comparison of effects of high- and low-frequency electromagnetic fields on proliferation and differentiation of neural stem cells.
- Source :
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Neuroscience Letters . Jan2021, Vol. 741, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
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Abstract
- • Transcranial magnetic stimulation (TMS) has been widely applied to diagnose and treat intractable brain diseases. • Both 50 Hz LF-EMF and HF-EMF can promote the proliferation of NSCs in vitro. • LF-EMF can accelerate NSCs to differentiate into neurons. To compare the effects of high- (HF-EMF) and low-frequency electromagnetic fields (LF-EMF) on the proliferation and differentiation of neural stem cells (NSCs). NSCs were obtained from SD rat hippocampus and cultured in suspension and adherent differentiation media. NSCs were exposed to LF-EMF (5 m T, 50 Hz, 30 min daily), HF-EMF (maximum magnetic induction 2.5 T, 40 % MO, 50 Hz, 10 min daily) and no electromagnetic field. At 3 d, cell viability and quantity of NSCs in suspension were detected by CCK-8 assay and cell counting plate. Immunofluorescence staining and qRT-PCR were performed to detect the percentage of Tu j -1 and GFAP-positive NSCs and the expression of Tuj-1 and GFAP mRNA. The P3 NSCs were positive with Nestin and induced NSCs expressed Tuj-1, GFAP and oligodendrocyte markers (MBP). CCK-8 assay and cell counting showed that the OD value and quantity of LF-EMF group were significantly higher than those in other two groups (both P < 0.05). Compared with the control group, the OD value and quantity were significantly higher in the HF-EMF group (P < 0.05). Immunofluorescence staining and qRT-PCR revealed that the percentage of Tuj-1 positive cells and the expression of Tuj-1 mRNA of NSCs exposed to LF-EMF were the highest (both P < 0.05). The proportion of GFAP-positive NSCs and the expression of GFAP mRNA did not significantly differ among three groups (all P > 0.05). Both 50 Hz LF-EMF and HF-EMF can promote the proliferation of NSCs in vitro and LF-EMF can accelerate NSCs to differentiate into neurons. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03043940
- Volume :
- 741
- Database :
- Academic Search Index
- Journal :
- Neuroscience Letters
- Publication Type :
- Academic Journal
- Accession number :
- 147649160
- Full Text :
- https://doi.org/10.1016/j.neulet.2020.135463