Back to Search
Start Over
Multiplane differential phase contrast imaging using asymmetric illumination in volume holographic microscopy.
- Source :
-
Journal of Biomedical Optics . Dec2020, Vol. 25 Issue 12, p123704-123704. 1p. - Publication Year :
- 2020
-
Abstract
- Significance: Differential phase contrast (DPC) is a well-known imaging technique for phase imaging. However, simultaneously acquiring multidepth DPC images is a non-trivial task. We propose simultaneous multiplane DPC imaging using volume holographic microscopy (VHM). Aim: To design and implement a new configuration of DPC-VHM for multiplane imaging. Approach: The angularly multiplexed volume holographic gratings (AMVHGs) and the wavelength-coded volume holographic gratings (WC-VHGs) are used for this purpose. To obtain asymmetric illumination for DPC images, a dynamic illumination system is designed by modifying the regular Köhler illumination using a thin film transistor panel (TFT-panel). Results: Multidepth DPC images of standard resolution chart and biosamples were used to compare imaging performance with the corresponding bright-field images. An average contrast enhancement of around three times is observed for target resolution chart by DPC-VHM. Imaging performance of our system is studied by modulation transfer function analysis, which suggests that DPC-VHM not only suppresses the DC component but also enhances high-frequency information. Conclusions: Proposed DPC-VHM can acquire multidepth-resolved DPC images without axial scanning. The illumination part of the system is adjustable so that the system can be adapted to bright-field mode, phase contrast mode, and DPC mode by controlling the pattern on the TFT-panel. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10833668
- Volume :
- 25
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Journal of Biomedical Optics
- Publication Type :
- Academic Journal
- Accession number :
- 147856820
- Full Text :
- https://doi.org/10.1117/1.JBO.25.12.123704