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miR-340 Exerts Suppressive Effect on Retinoblastoma Progression by Targeting KIF14.
- Source :
-
Current Eye Research . Feb2021, Vol. 46 Issue 2, p232-238. 7p. - Publication Year :
- 2021
-
Abstract
- Purpose: This work aimed to investigate the influences of microRNA-340 (miR-340) on proliferation and apoptosis of retinoblastoma (RB) cells and explore its regulatory mechanism. miR-340 mimic and inhibitor were applied for up-regulating or inhibiting the expression of miR-340 in RB cell lines. Then, CCK-8 and AnnexinV-FITC/PI staining were used to measure cell proliferation and apoptosis, respectively. After that, luciferase assay was performed to affirm the direct targets of miR-340. Furthermore, qRT-PCR and western blotting assay were carried out to detect the levels of miR-340 and KIF14. Our results indicated that the miR-340 was lowly expressed in RB cell lines, and up-regulation of miR-340 can decrease the proliferation and induce the apoptosis of RB cells. Moreover, we verified that miR-340 controls KIF14 expression, either directly or through a subsequent molecular cascade, and inversely related to its expression. The results obtained from the rescue assays presented that over-expression of KIF14 reversed the miR-340-mediated inhibition on malignant phenotype of RB cells. Overall, we proved that miR-340 can decrease the proliferation and increase the apoptosis of RB cells, and its function in RB cells was at least partially achieved via down-regulation of KIF14, prompting that miR-340 was expected to supply a new direction for clinical therapy of RB in the future. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02713683
- Volume :
- 46
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Current Eye Research
- Publication Type :
- Academic Journal
- Accession number :
- 147965353
- Full Text :
- https://doi.org/10.1080/02713683.2020.1795202