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Housefly Phormicin inhibits Staphylococcus aureus and MRSA by disrupting biofilm formation and altering gene expression in vitro and in vivo.
- Source :
-
International Journal of Biological Macromolecules . Jan2021, Vol. 167, p1424-1434. 11p. - Publication Year :
- 2021
-
Abstract
- The increasing drug resistance of pathogenic bacteria is a crisis that threatens public health. Antimicrobial peptides (AMPs) have been suggested to be potentially effective alternatives to solve this problem. Here, we tested housefly Phormicin-derived peptides for effects on Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) infections in vitro and in vivo. A decreased bacterial load of MRSA was observed in the mouse scald model after treatment with Phormicin and in the positive control group (vancomycin). A mouse scrape model indicated that Phormicin helps the host fight drug-resistant MRSA infections. The protective effect of Phormicin on MRSA was confirmed in the Hermetia illucens larvae model. Phormicin also disrupted the formation of S. aureus and MRSA biofilms. Furthermore, this effect coincided with the downregulation of biofilm formation-related gene expression (agrC , sigB , RNAIII , altA , rbf , hla , hld , geh and psmɑ). Notably, virulence genes and several regulatory factors were also altered by Phormicin treatment. Based on these findings, housefly Phormicin helps the host inhibit MRSA infection through effects on biofilm formation and related gene networks. Therefore, housefly Phormicin potential represents a candidate agent for clinical MRSA chemotherapy. Unlabelled Image • Phormicin effectively inhibits MRSA infection in skin scratch or scald mice model and promotes the healing of scald wounds. • Phormicin improves the survival of Hermetia illucens larvae infected with MRSA in vivo. • Phormicin inhibits biofilm formation of Staphylococcus aureus and MRSA. • Phormicin altered gene expression of MRSA biofilm formation and virulence. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 167
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 147992755
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2020.11.096