慢病毒介导MicroRNA-376b-3p 对垂体腺瘤细胞增殖和凋亡的 影响及机制探究.

Objective: To investigate the effect of lentivirus-mediated microrna-376b-3p on the proliferation and apoptosis of pituitary adenomas and its possible mechanism. Methods: Taking human pituitary adenoma cell lines cultured in vitro as the research object, the microRNA-376b-3p mimics group (control group) and lenti-sh MicroRNA-376b-3p group (drug group) were established respectively, and the MTT method was used to detect MicroRNA-376b-3p on the proliferation of pituitary adenoma cells, the AnnexinV-FITC/PI double staining method was used to detect the effect of MicroRNA-376b-3p on the apoptosis rate of pituitary adenoma cells, and the Western blot method was used to detect the effect of MicroRNA-376b-3p on HMGA2, Bax, Caspase-3 protein expression. Result: (1) Lentivirus-mediated Microrna-376b-3p could significantly inhibit the proliferation of pituitary adenoma cells (P<0.05). (2) At 12, 24 and 48 h after microrna-376b-3p intervention, the apoptosis rate of pituitary gland cells in the control group was significantly lower than that in the drug group (P<0.05). Conclusions: (3) After transfection with microrna-376b-3p transformation, the expression of Bax protein increased, and the expression of Bcl-2, Caspase-3, Survivin and HMGA2 protein decreased (P<0.05). Lentivirus-mediated Microrna-376b-3p could significantly inhibit the proliferation of pituitary adenoma cells and induce apoptosis. The mechanism of microrna-376b-3p may be related to its targeted down-regulation of HMGA2 expression, up-regulation of Bax protein expression and regulation of survivin protein expression. [ABSTRACT FROM AUTHOR]