High Occurence of Methicillin Resistant Staphylococcus sciuri (MRSS) and First Detection of mecC from Broiler Flocks in Turkey.

In this study, twelve broiler farms were randomly sampled in three different cities (Adana, Hatay and Sakarya) in Turkey, to investigate the presence and genetic diversity of methicillin resistant coagulase-negative staphylococci (MRCoNS). Four houses for each broiler flock were selected for sampling and one hundred throat swabs from each house, from each broiler flock were collected and divided into five pools of 20 swabs. Overall, 63 methicillin resistant S. sciuri were isolated, and mecA was alone detected in 56 isolates, seven isolates were positive not only for mecA, but also for mecC. Most of the isolates (77.8%) were non-typeaple for SCCmec and ten isolates (15.9%) carried SCCmec type III. All isolates were resistant to cefoxitin and penicillin, and the frequency of the isolates for trimethoprim/sulfamethoxazole, clindamycin, erythromycin, tetracycline resistance ranged between 66.7% and 73%. The PFGE analysis revealed the presence of 47 distinct pulsotypes. The most common resistant genes detected were lnuA and tet genes. None of the isolates was positive for toxin and disinfectant resistance genes. The present study is the first reporting the presence of the mecC gene among CoNS in Turkey. The results of this study also indicated that broiler flocks were potential source of multi-resistant methicillin resistant S. sciuri, with a high diversity. [ABSTRACT FROM AUTHOR]