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Interaction among estrogen, IGF-1, and H2S on smooth muscle cell proliferation.
- Source :
-
Journal of Endocrinology . Jan2021, Vol. 248 Issue 1, p17-30. 14p. - Publication Year :
- 2021
-
Abstract
- Both estrogen and hydrogen sulfide (H2S) inhibit the proliferation of vascular smooth muscle cells (SMCs) and development of atherosclerosis. In the absence of endogenous H2S as occurred in CSE-knockout (KO) mouse, however, estrogen stimulates the proliferation of vascular SMCs. The underlying mechanisms for this seemingly controversial vascular effect of estrogen are unclear. In the present study, we demonstrated that the stimulatory effect of estrogen on the p roliferation of CSE-KO SMCs was suppressed by the inhibitor of insulin-like growth fac tor-1 receptor (IGF-1R) or knockdown of IGF-1R protein expression. Estrogen downregulat ed the expression of insulin-like growth factor-1 (IGF-1) and IGF-1R in aortic tissu es or aortic SMCs isolated from WT and CSE-KO mice. Furthermore, endogenous H2S downregulated IGF-1R, but upregulated estrogen receptor (ER)-α, in aortic tissues or SMCs. ER-α and IGF-1R were co-located in SMCs and co-immunoprecipitated, which was decreas ed by H2S. Finally, both endogenous and exogenous H2S induced the S-sulfhydration of IGF-1R, but not ER-α, in WT-SMCs and CSE-KO SMCs, which underlies the decreased for mation of IGF-1R/ER-α hybrid in the presence of H2S. Thus, the absence of H2S favors the interaction of estrogen with IGF-1R/ER-α hybrid to stimulate SMCs proliferation. The appreciation of a critical role of H2S in preventing estrogen-induced SMCs proliferation will help b etter understand the regulation of complex vascular effects of estroge n and sex-related cardiovascular diseases. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00220795
- Volume :
- 248
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 148052778
- Full Text :
- https://doi.org/10.1530/JOE-20-0190