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Protective effect of hyperoside against hydrogen peroxide-induced dysfunction and oxidative stress in osteoblastic MC3T3-E1 cells.
- Source :
-
Artificial Cells, Nanomedicine & Biotechnology . Dec2020, Vol. 48 Issue 1, p377-383. 7p. - Publication Year :
- 2020
-
Abstract
- Oxidative stress can induce apoptosis and decrease activities of osteoblasts. Hyperoside (HYP) is a potent antioxidant derived from Chinese herb. This study aims to evaluate the protective effects provided by HYP to osteoblastic MC3T3-E1 cells. MC3T3-E1 cells were pre-treated with HYP for 24 h before being treated with 0.3 mM hydrogen peroxide (H2O2) for 24 h. Cell viability, flow cytometric analysis and mRNA expression of alkaline phosphatase (ALP), collagen I (COL-I) and osteocalcin (OCN) in MC3T3-E1 cells were examined. We next examined apoptosis-related and mitogen-activated protein kinase (MAPK) related proteins in HYP and H2O2 groups. HYP over the dose of 40 μmol/L could obviously increase the MC3T3-E1 cell viability at 24 h and 48 h (p <.05). HYP significantly (p <.05) increased mRNA expression of ALP, COL-I and OCN than H2O2 group. Moreover, HYP decreased the apoptosis rate and apoptosis-related proteins that induced by H2O2. In addition, HYP decreased the production of phosphorylated Jun N-terminal kinase (JNK) and p38 levels of osteoblastic MC3T3-E1 cells induced by H2O2. These results demonstrated that the protective effect provided by HYP to osteoblastic MC3T3-E1 cells was mediated, at least in part, via inhibition of MAPK signalling pathway and oxidative damage of the cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 21691401
- Volume :
- 48
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Artificial Cells, Nanomedicine & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 148164691
- Full Text :
- https://doi.org/10.1080/21691401.2019.1709851