Fluorescent Fluoride Sensor Based on Indolizine Core Skeleton for Bioimaging.

Keywords: Fluorescent probe; Indolizine; Fluoride sensor; Bioimaging EN Fluorescent probe Indolizine Fluoride sensor Bioimaging 95 98 4 01/29/21 20210101 NES 210101 Schematic representation of fluoride anion sensing with Silyl-KIz in live cell GLO:1CJF/01jan21:bkcs12134-toc-0001.jpg PHOTO (COLOR): . gl Fluoride ions are used widely in toothpaste, pharmaceutical agents, and even drinking water for the prevention of dental caries and the amelioration of osteoporosis.1 However, a concentration of fluoride above 1.5 mg/mL (79 M) in drinking water can cause dental and/or skeletal fluorosis, as defined by the WHO.2 Therefore, there is a strong demand for new methods for detecting fluoride ions.3 Although numerous analytical methods have been developed for fluoride sensing, including mass spectrometry, atomic absorption spectrophotometry, ion-selective electrode analysis, nuclear magnetic resonance (NMR) spectroscopy, and ion chromatography,4 the requirement for inconvenient equipment and/or sophisticated procedures limits the accessibility of the analysis.5 To overcome these limitations, researchers have continued to explore the development of fluorescent probes for fluoride ions.6-8 However, it is still a challenge to develop a practical fluorescent probe for these ions in live cells. To confirm that Silyl-KIz could be used as a fluorescent fluoride sensor, the emission intensity of the Silyl-KIz solution in 1× PBS in the presence of different fluoride ion concentrations was measured (Figure 3). To demonstrate the suitability of Silyl-KIz for biological applications, we investigated whether Silyl-KIz could sensitively detect fluoride ions in live HeLa cells. [Extracted from the article]