Metabolic profiling reveals alterations in the erythrocyte response to fava bean ingestion in G6PD‐deficient mice.

BACKGROUND Favism is an acute hemolytic syndrome caused by fava bean (FB) ingestion. The purpose of this study was to investigate the possible influences of FB on the metabonomic profile of erythrocytes in glucose‐6‐phosphate dehydrogenase (G6PD)‐deficient (G6PDx) and wild‐type (WT) mice. RESULTS: Ninety‐two metabolites were identified in the comparison of the G6PDx and WT groups. Eighty‐seven metabolites were identified in the erythrocytes of WT and G6PDx mice after FB ingestion. Thirty‐eight metabolites were identified in the comparison of the FB‐treated G6PDx and the FB‐treated WT mouse groups. Among them, the number of glycerophospholipids (GPLs) and polyunsaturated fatty acids (PUFAs) changed significantly, which suggests that GPLs and PUFAs may be responsible for FB stress. CONCLUSION: This study demonstrates that G6PD deficiency might affect the metabonomic profile of erythrocytes in response to FB. © 2020 Society of Chemical Industry [ABSTRACT FROM AUTHOR]