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Separation and purification of horseradish peroxidase from horseradish roots using a novel integrated method.
- Source :
-
New Journal of Chemistry . 1/28/2021, Vol. 45 Issue 4, p1959-1966. 8p. - Publication Year :
- 2021
-
Abstract
- The aim of the present work was to develop a novel method integrating two-step aqueous two-phase extraction and temperature-controlled affinity precipitation for the separation and purification of horseradish peroxidase (HRP) from horseradish roots. First, a two-step aqueous two-phase extraction (ATPE) process was used as a primary separation process to remove sugars and pigments, and then a temperature-controlled affinity precipitation process based on a thermosensitive triblock copolymer (PEG113-b-PVBA49-b-PNIPAM105) was exploited as a further purification process to selectively isolate HRP from other impurity proteins. The optimum PEG4000 (0.20, w/w) − (NH4)2SO4 (0.18, w/w) aqueous two-phase system (ATPS) was obtained by studying the liquid–liquid equilibrium of PEG (400/1000/2000/4000) + (NH4)2SO4/K3C6H5O7/K2HPO4 ATPSs and the distribution behaviors of 9 kinds of model compounds in these PEG-salt ATPSs. The successful isolation of HRP from horseradish roots was achieved by the integrated method without having a negative effect on the structure of HRP, and the extraction efficiency and purification factor of HRP reached 84.26 ± 0.15% and 9.15 ± 0.10, respectively. The integrated method shows outstanding separation and purification performances and has good prospects for the industrial production of plant glycoprotein. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 11440546
- Volume :
- 45
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- New Journal of Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 148435615
- Full Text :
- https://doi.org/10.1039/d0nj04614k