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Engineering Yarrowia lipolytica for sustainable production of the chamomile sesquiterpene (−)-α-bisabolol.
- Source :
-
Green Chemistry . 1/21/2021, Vol. 23 Issue 2, p780-787. 8p. - Publication Year :
- 2021
-
Abstract
- The sesquiterpene (−)-α-bisabolol has been shown to have antibacterial, antiseptic, and anti-inflammatory activities, as well as skin-soothing properties, which has led to its wide use in cosmetics. However, the current production of (−)-α-bisabolol via steam-distillation of plants is not sustainable and cannot meet the increasing market demand. In the present study, a sustainable strategy for (−)-α-bisabolol production was developed by constructing a Yarrowia lipolytica cell factory. The oleaginous yeast Y. lipolytica has a native mevalonate pathway and strong acetyl-CoA flux, which can supply adequate precursors for (−)-α-bisabolol production. The endogenous mevalonate pathway and heterologous (−)-α-bisabolol synthesis pathway were optimized by strengthening the rate-limiting enzymes, downregulating competing pathway, as well as balancing the distribution of the common precursor acetyl-CoA between endogenous lipids and heterologous (−)-α-bisabolol synthesis by strengthening the β-oxidation pathway. Finally, (−)-α-bisabolol production of up to 364.23 mg L−1 was achieved in batch culture. This sustainable method has potential for industrial (−)-α-bisabolol production. [ABSTRACT FROM AUTHOR]
- Subjects :
- *INDUSTRIAL capacity
*ACETYLCOENZYME A
*LIPIDS
*ENZYMES
Subjects
Details
- Language :
- English
- ISSN :
- 14639262
- Volume :
- 23
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Green Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 148436114
- Full Text :
- https://doi.org/10.1039/d0gc03180a