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Biofabrication of silver nanoparticles using bacteria from mangrove swamp.

Biofabrication of silver nanoparticles using bacteria from mangrove swamp.

Authors :
Sharma, Manish
Nayak, Parth Sarthi
Asthana, Shreyasi
Mahapatra, Dipankar
Arakha, Manoranjan
Jha, Suman
Source :
IET Nanobiotechnology (Wiley-Blackwell). Aug2018, Vol. 12 Issue 5, p626-632. 7p.
Publication Year :
2018

Abstract

The last decade has observed a rapid advancement in utilising biological system towards bioremediation of metal ions in the form of respective metal nanostructures or microstructures. The process may also be adopted for respective metal nanoparticle biofabrication. Among different biological methods, bacteria‐mediated method is gaining great attention for nanoparticle fabrication due to their eco‐friendly and cost‐effective process. In the present study, silver nanoparticle (AgNP) was synthesised via continuous biofabrication using Aeromonas veronii, isolated from swamp wetland of Sunderban, West Bengal, India. The biofabricated AgNP was further purified to remove non‐conjugated biomolecules using size exclusion chromatography, and the purified AgNPs were characterised using UV–visible spectroscopy, X‐ray diffraction, field emission scanning electron microscopy and transmission electron microscopy (TEM). Additionally, the presence of proteins as capping and stabilising agents was confirmed by the amide‐I and amide‐II peaks in the spectra obtained using attenuated total reflection Fourier transform infrared spectroscopy. The size of biofabricated AgNP was 10–20 nm, as observed using TEM. Additionally, biofabricated AgNP shows significant antibacterial potential against E. coli and S. aureus. Hence, biofabricated AgNP using Aeromonas veronii, which found resistant to a significant concentration of Ag ion, showed enhanced antimicrobial activity compared to commercially available AgNP. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
17518741
Volume :
12
Issue :
5
Database :
Academic Search Index
Journal :
IET Nanobiotechnology (Wiley-Blackwell)
Publication Type :
Academic Journal
Accession number :
148455542
Full Text :
https://doi.org/10.1049/iet-nbt.2017.0205