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miR-296-5p Inhibits the Secretion of Pulmonary Surfactants in Pulmonary Epithelial Cells via the Downregulation of Wnt7b/ß-Catenin Signaling.
- Source :
-
BioMed Research International . 1/5/2021, Vol. 2021, p1-8. 8p. - Publication Year :
- 2021
-
Abstract
- Neonatal respiratory distress syndrome (NRDS) is a common disease that occurs in premature infants. However, the mechanisms underlying the disease remain unclear. microRNAs (miRNAs) have been indicated to play a crucial role in the development of NRDS. In this study, we aimed to explore the regulatory mechanisms of miR-296-5p in NRDS. The expression levels of miR-296-5p in preterm infants with NRDS were determined using quantitative reverse-transcription polymerase chain reaction (RTqPCR). A549 cells were transfected with lentiviral vectors encoding miR-296-5p, and the transfection efficiency was determined using RT-qPCR. Flow cytometry and CCK8 assay were performed to measure apoptosis and proliferation of A549 cells, respectively. The protein levels of pulmonary surfactant SP-A (SFTPA1), SP-B, Wnt7b, and ß-catenin were measured using western blotting. We demonstrated an upregulation of miR-296-5p in NRDS. The miR-296-5p was successfully overexpressed in A549 cells via lentivirus transfection, and the upregulation of miR-296-5p inhibited cell proliferation and secretion of SP-A and SP-B and also induced downregulation of the Wnt7b/ß-catenin in vitro. Therefore, miR-296-5p inhibits cell proliferation and secretion of pulmonary surfactants in A549 cells via downregulation of Wnt7b/ß-catenin signaling. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CELL proliferation
*DISEASE vectors
*APOPTOSIS
*BIOLOGICAL assay
*CELL lines
*CELLULAR signal transduction
*CHOLECYSTOKININ
*CYTOSKELETAL proteins
*EPITHELIAL cells
*FLOW cytometry
*GENETIC techniques
*LUNGS
*POLYMERASE chain reaction
*PULMONARY surfactant
*RESPIRATORY distress syndrome
*RETROVIRUSES
*WESTERN immunoblotting
*WNT proteins
*REVERSE transcriptase polymerase chain reaction
*MICRORNA
*IN vitro studies
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Volume :
- 2021
- Database :
- Academic Search Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 148462473
- Full Text :
- https://doi.org/10.1155/2021/4051504