Back to Search
Start Over
Enhancement of nitrite-dependent anaerobic methane oxidation via Geobacter sulfurreducens.
- Source :
-
Science of the Total Environment . Apr2021, Vol. 766, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- Nitrite-dependent anaerobic methane-oxidation (n-damo) is a potential novel technology for nitrogen removal in anaerobic wastewater treatment. In this study, Geobacter sulfurreducens (G) was applied to stimulate n-damo activity. Conductive materials such as nano-magnetite (M) or aggregating agents such as hydroxylapatite (H) were co-added with G. sulfurreducens to further investigate the enhancement effect. Results showed that the nitrite reduction activity of the n-damo culture was promoted by G. sulfurreducens , with 1.71–2.38 times higher in treatment G, G + M, and G + H than that in the control, but was inhibited by the single addition of hydroxylapatite. N-damo bacterial abundances based on the qPCR of the n-damo-specific pmoA gene increased in treatments with G. sulfurreducens , compared with that of the control. High-throughput sequencing analysis revealed the enrichment of uncultured phylum WPS-2 in treatments with G. sulfurreducens. Fluorescence in situ hybridization verified the co-occurrence pattern of n-damo bacteria (NC10), G. sulfurreducens , and type-I aerobic methanotrophs (Methylomonas spp.). The above results corroborated the microbial interspecies electron transfer (MIET) potentiality of the n-damo enrichment. Our study provides a novel pathway for enhancing MIET to stimulate n-damo process. Unlabelled Image • N-damo was enhanced via application with Geobacter sulfurreducens. • Co-adding of G. sulfurreducens and hydroxylapatite enhanced the most significantly. • Uncultured WPS-2 was selectively enriched by addition with G. sulfurreducens. • Co-existence of NC-10, Geobacter , and Methylomonas was detected. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00489697
- Volume :
- 766
- Database :
- Academic Search Index
- Journal :
- Science of the Total Environment
- Publication Type :
- Academic Journal
- Accession number :
- 148634907
- Full Text :
- https://doi.org/10.1016/j.scitotenv.2020.144230