甜菜碱通过IGF-1R/ β-catenin 信号通路介导对２ 型糖 尿病大鼠骨流失的保护作用.

To investigate the effect of betaine ( TCJ ) on bone loss in type 2 diabetic rats and explore the possible mechanisms. Methods Thirty rats were randomly divided into a control group (CON), a model group (MOD), and a betaine group ( TCJ ), 10 in each group. Among them, type 2 diabetes models were established for MOD and TCJ group. Then TCJ group rats received 50 mg per day I kg betaine treatment for 12 weeks; after the treatment, they were detected by Micro-CT, HE stained sections and Western blot. Results After 12 weeks of treatment, compared with the MOD group, the result of the three-point bending test, Micro-CT and HE staining showed that the number of trabeculae, bone strength and bone density of the TCJ group were significantly improved(P<0.05).The maximum load and elastic modulus, BMD, TV/BV, Th.N, Tb.Th and Tb.Sp of the TCJ group were significantly improved compared with the MOD group ( P<O. 05). Compared with the MOD group, the expression level of r1>-catenin in the TCJ group was significantly increased, while the expression levels ofp-IGF-lR, p-GSK-3r1>, p-r1>-catenin, IGF-lR and GSK-3~ were significantly reduced ( P<O. 05). Conclusion Betaine mediates the protective effect on bone loss in type 2 diabetic rats through IGF-lR I ~-catenin signaling pathway. [ABSTRACT FROM AUTHOR]