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Chimeric ferritin H in hybrid crucian carp exhibits a similar down-regulation in lipopolysaccharide-induced NF-κB inflammatory signal in comparison with Carassius cuvieri and Carassius auratus red var.
- Source :
-
Comparative Biochemistry & Physiology Part C: Toxicology & Pharmacology . Mar2021, Vol. 241, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
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Abstract
- Ferritin H can participate in the regulation of teleostean immunity. ORF sequences of RCC/WCC/WR-ferritin H were 609 bp, while WR-ferritin H gene possessed chimeric fragments or offspring-specific mutations. In order to elucidate regulation of immune-related signal transduction, three fibroblast-like cell lines derived from caudal fin of red crucian carp (RCC), white crucian carp (WCC) and their hybrid offspring (WR) were characterized and designated as RCCFCs, WCCFCs and WRFCs. A sharp increase of ferritin H mRNA was observed in RCCFCs, WCCFCs and WRFCs following lipopolysaccharide (LPS) challenge. Overexpression of RCC/WCC/WR-ferritin H can decrease MyD88-IRAK4 signal and antagonize NF-κB, TNFα promoter activity in RCCFCs, WCCFCs and WRFCs, respectively. These results indicated that ferritin H in hybrid offspring harbors highly-conserved domains with a close sequence similarity to those of its parents, playing a regulatory role in inflammatory signals. Unlabelled Image • We characterized fin cell lines derived from hybrid crucian carp and its parents. • Structure of chimeric ferritin H in hybrid offspring was compared with those of its parents. • Up-regulated expressions of RCC/WCC/WR-ferritin H were observed following LPS stimulation. • Overexpression of RCC/WCC/WR-ferritin in fish cells can abrogate NF-κB promoter activity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15320456
- Volume :
- 241
- Database :
- Academic Search Index
- Journal :
- Comparative Biochemistry & Physiology Part C: Toxicology & Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 148777573
- Full Text :
- https://doi.org/10.1016/j.cbpc.2020.108966