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Immobilization of Lepidium draba peroxidase on a novel Zn-MOF nanostructure.

Authors :
Farhadi, Soudabeh
Riahi-Madvar, Ali
Sargazi, Ghasem
Mortazavi, Mojtaba
Source :
International Journal of Biological Macromolecules. Mar2021, Vol. 173, p366-378. 13p.
Publication Year :
2021

Abstract

In the present study, ultrasound irradiation was utilized to synthesize a novel zinc metal-organic framework (MOF). Scanning electron microscopic images, exhibited homogenous morphology with a nano-sized distribution of the Zn-MOF structure as also confirmed by X-ray diffraction patterns. Following, physical immobilization of Lepidium draba peroxidase (LDP) were optimized on the Zn-MOF in phosphate buffer (50 mM, pH 6.5), ratio amount of MOF/enzyme; 7/1 after shaking for 15 min at 25 °C, with high protein loading of 109.9 mg/g and immobilization yield of 93.3%. Immobilized enzyme (IE) exhibited more than 330% enhanced specific activity and also exhibited more than 150% specific affinity to its substrate (3,3′,5,5′-tetramethylbenzidine) with respect to the free enzyme (FE). Optimum temperature of the IE was obtained at 20 °C while its was 25 °C for the FE, and thermostability of the IE augmented at temperature of 30 °C and 40 °C by the factors of 104 and 108% respectively. pH stability under neutral and basic condition and storage stability of the IE improved with respect to the FE as well as its structural stability (T m ; 73 °C for IE vs. 63 °C for FE). Furthermore, immobilization is accompanied with alteration on the enzyme structure as revealed by the intrinsic and extrinsic fluorescence spectra. • New Zn-MOF nanostructure was synthesized using ultrasound irradiations. • Recombinant LDP was successfully immobilized on the nanostructure. • Specific activity, storage stability and structural stability of the enzyme were improved upon immobilization. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01418130
Volume :
173
Database :
Academic Search Index
Journal :
International Journal of Biological Macromolecules
Publication Type :
Academic Journal
Accession number :
148929972
Full Text :
https://doi.org/10.1016/j.ijbiomac.2020.12.216