Metabolomics of potential contenders conferring antioxidant property to varied polar and non-polar solvent extracts of Edgaria darjeelingensis C.B.Clarke.

Background: The Himalaya hotspot, habitat to numerous indigenous species, harbours an endemic and endangered, cucurbit climber Herpetospermum darjeelingense (C.B.Clarke) H.Schaef. & S.S.Renner [Edgaria darjeelingensis C.B.Clarke]. Seeds of this plant have traditionally been used for ethno-veterinary treatment. The present probe aims to provide an insight into the antioxidant-related biochemical attributes of this phytochemically unexplored plant. Results: DPPH scavenging activity of solvent extracts showed more potential towards the polar end with its maximum activity in methanolic extract (84.64%). Acetone extract showed more ferric reducing power than the ethanol fractions. Maximum flavonoid quantification was recorded in ethanol extract. GC–MS of methanolic extract detected 39 compounds of which 22 have preventive and curing properties for several ailments. Thirteen compounds were reported with antioxidant activity and cover an area of 63.92% among the total detected compounds by GC–MS. 1,3,4,5-Tetrahydroxy-cyclohexanecarboxylic acid; Hexadecanoic acid, methyl ester; ß sitosterol and Phytol are present in significant quantity and are the prime candidates conferring antioxidant potential. Chromatographically fractionalized methanol extract with non-polar to polar solvents and their subsequent DPPH scavenging analysis exhibited a major peak in ethanol fraction due to the presence of Phytol in considerable high quantity and some other compounds. Further sub-fractionation of ethanolic fraction provides a supportive evidence of ethanol solubility of the compounds. Biosynthetic lineage reveals fatty acid synthesis, intermediates of Shikimate pathway and terpenoid metabologenesis schemes lead to the several antioxidant molecules detected in our extract. Conclusion: Metabolomics of antioxidants will provide a better understanding in its intracellular free radical quenching activity and isolation of these bioactive compounds can boost pharmaceutical industry. [ABSTRACT FROM AUTHOR]