Effects and interaction of dietary electrolyte balance and citric acid on growth performance, intestinal histomorphology, digestive enzyme activity and nutrient transporters expression of weaned piglets.

Fifty‐six piglets were weaned at 21 days and randomly assigned to 1 of 8 dietary treatments with 7 replicate pens for a 14‐day experimental period. The eight experimental diets were prepared via a 2 × 4 factorial arrangement with citric acid (CA; 0 and 0.3%) and dietary electrolyte balance (dEB, Na +K − Cl mEq/kg of the diet; −50, 100, 250, and 400 mEq/kg). Varying dEB values were obtained by altering calcium chloride and sodium bicarbonate contents. Dietary CA significantly increased (p <.05) villus height (VH) and villus height:crypt depth (VH:CD) in the jejunum. Piglets fed a 250 mEq/kg diet increased (p <.05) VH and VH:CD values in the duodenum. Jejunal VH and VH:CD increased (quadratic; p <.05), and ileal VH:CD (liner and quadratic; p <.05) decreased as dEB was increased in diets without CA, but no such effect was observed on the diets containing CA (dEB ×CA; p <.05). The CD in jejunum (quadratic; p <.05) increased as dEB was increased in diets containing CA, whereas it was decreased (linear; p <.05) in the diets without CA (dEB ×CA; p <.001). Dietary CA increased maltase activity and reduced the number of Ki67‐positive cells (p <.05). Increasing dEB values in diets without CA increased sucrose and lactase activities (quadratic; p <.05), but no such effect was observed in the diets with CA (dEB ×CA; p <.05). An interaction effect between dEB and CA on the number of Ki67‐positive cells was observed (p <.001). In conclusion, 250 mEq/kg dEB diet with CA improved piglet intestinal digestion and absorption function by improving intestinal morphology and increasing digestive enzyme activities. However, these improvements were also observed in piglets fed the 100 mEq/kg dEB diet without CA. [ABSTRACT FROM AUTHOR]