Near-infrared fluorescent probe for rapid detecting H2S and its application in nanofibrous film and living cells.

Hydrogen sulfide (H 2 S) is an important endogenous gaseous signal transmitter. In this work, a novel near-infrared fluorescent probe DS for the detection of H 2 S was designed and synthesized based on the theoretical analysis. The optical investigation results showed that H 2 S could be detected quantitatively in the concentration range 0.0–10.0 μM, and the detection limit was calculated to be 9.0 nM (fluorescence intensity) and 88.2 nM (absorbance). Furthermore, probe DS was successfully applied for imaging H 2 S in living cells, which exhibited the admirable advantages of membrane permeability and biocompatibility. Moreover, a portable fluorescence nanofibrous film prepared by electrospinning was successfully constructed for the visual detection of H 2 S under the UV lamp, indicating its potential applications in the area of portable membrane sensing. Image 1 • A novel near-infrared fluorescent probe based on DCM scaffolds for H 2 S was developed. • The probe displayed the detection limit of 9.0 nM for H 2 S. • The probe was successfully applied for imaging H 2 S in living cells. • The probe showed potential applications in the area of portable membrane sensing. [ABSTRACT FROM AUTHOR]