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A chromene based fluorescence probe: Accurate detection of peroxynitrite in mitochondria, not elsewhere.
- Source :
-
Sensors & Actuators B: Chemical . May2021, Vol. 334, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- • A novel fluorescent probe was synthesized for the detection of ONOO− by conjugating dicyano-vinyl with benzopyran-chromone. • The probe shows specifically selectivity over other biologically relevant ROS/RNS/RSS. • The sensing mechanism is the cleavage of the carbon-carbon double bond by the nucleophilicity and oxidation of peroxynitrite. • The probe has a certain mitochondrial targeting and localization effect. • BC-PN-2 can accurately identify ONOO− in mitochondria by imaging of exogenous ONOO− in HepG2 cells. A novel fluorescent probe (BC-PN-2) for the detection of ONOO− was synthesized by conjugating dicyano-vinyl with benzopyran-chromone. The dicyano-vinyl group can be destructed by ONOO− to generate an aldehyde group resulting in strong fluorescence. Compared with the general fluorescence probes, a novel reactive site to detect ONOO− in mitochondria is used in this probe and the results show short response time, high sensitivity and excellent selectivity toward ONOO− among reactive oxygen species (ROS) and reactive nitrogen species (RNS) in PBS. Subsequent fluorescence imaging shows the high membrane permeability, a certain mitochondrial targeting and localization effect of the probe. In addition, the product BC-aldehyde cannot permeate through the membrane. Meanwhile, BC-PN-2 can accurately identify ONOO− in mitochondria by imaging of exogenous ONOO− in HepG2 cells and prove detected ONOO− are from mitochondria. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 334
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 149220354
- Full Text :
- https://doi.org/10.1016/j.snb.2021.129603