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Development of a monoclonal antibody for the detection of xylazine in milk and its use in an immunochromatographic strip.
- Source :
-
New Journal of Chemistry . 3/14/2021, Vol. 45 Issue 10, p4658-4665. 8p. - Publication Year :
- 2021
-
Abstract
- We prepared a monoclonal antibody (mAb) which was sensitive and specific against xylazine based on a new hapten. The 50%-inhibitory concentration (IC50) of the mAb against xylazine was 0.399 ng mL−1 and the mAb was of the IgG2b subclass. A cross-reactivity test showed that the mAb had very high specificity for xylazine. An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and gold nanoparticle-based lateral-flow test (GNT) strip were developed for the rapid screening of xylazine in milk samples. The limit of detection (LOD) and cut-off value of the GNT assay were evaluated to be 20 and 200 ng mL−1 in milk samples with the naked eye. The average recoveries of xylazine which was detected by the ic-ELISA ranged from 95.1% to 105.1%. Real samples were used to confirm the accuracy of the developed GNT strip. These results demonstrated that we had developed a very reliable method for rapid detection of xylazine in milk samples. [ABSTRACT FROM AUTHOR]
- Subjects :
- *XYLAZINE
*ENZYME-linked immunosorbent assay
*MONOCLONAL antibodies
*MILK
Subjects
Details
- Language :
- English
- ISSN :
- 11440546
- Volume :
- 45
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- New Journal of Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 149295132
- Full Text :
- https://doi.org/10.1039/d0nj05810f