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Responsive Emulsions for Sequential Multienzyme Cascades.
- Source :
-
Angewandte Chemie International Edition . 4/6/2021, Vol. 60 Issue 15, p8410-8414. 5p. - Publication Year :
- 2021
-
Abstract
- Multienzyme cascade biocatalysis is an efficient synthetic process, avoiding the isolation/purification of intermediates and shifting the reaction equilibrium to the product side.. However, multienzyme systems are often limited by their incompatibility and cross‐reactivity. Herein, we report a multi‐responsive emulsion to proceed multienzyme reactions sequentially for high reactivity. The emulsion is achieved using a CO2, pH, and thermo‐responsive block copolymer as a stabilizer, allowing the on‐demand control of emulsion morphology and phase composition. Applying this system to a three‐step cascade reaction enables the individual optimal condition for each enzyme, and a high overall conversion (ca. 97 % of the calculated limit) is thereby obtained. Moreover, the multi‐responsiveness of the emulsion allows the facile and separate yielding/recycling of products, polymers and active enzymes. Besides, the system could be scaled up with a good yield. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14337851
- Volume :
- 60
- Issue :
- 15
- Database :
- Academic Search Index
- Journal :
- Angewandte Chemie International Edition
- Publication Type :
- Academic Journal
- Accession number :
- 149551370
- Full Text :
- https://doi.org/10.1002/anie.202013737