Simultaneous authentication of species identity and geographical origin of shrimps: Untargeted metabolomics to recurrent biomarker ions.

• Solving multiple seafood fraud issue by single untargeted metabolomics experiment. • Chemometrics models for predicting species and geographical origin of shrimp. • Identification of reproducible shrimp species specific marker ions. • Targeted tandem mass spectrometry method for rapid authentication of shrimp species. Mandatory disclosure of the species identity, production method, and geographical origin are embedded in the regulations and traceability systems, governing international seafood trade. A high-resolution mass spectrometry-based metabolomics approach could simultaneously authenticate the species identity and geographical origin of commercially important shrimps. The highly innovative approach spared the need for multiple testing methods which are in routine use currently. A robust chemometric model, developed using the metabolite fingerprint dataset, could accurately predict the species identity of the shrimp samples. Subsequently, species-specific biomarkers were discovered and a tandem mass spectrometry method for authentication of the species was developed. Two other chemometric models from the metabolomics experiment accurately predicted the geographical origin of king prawns and tiger prawns. The study has shown for the first time that food-metabolomics along with chemometrics can simultaneously check for multiple seafood fraud issues in the global seafood supply-chain. [ABSTRACT FROM AUTHOR]