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Oxidative stress plays a role in diabetes-induced bladder dysfunction in a rat model
- Source :
-
Urology . Nov2004, Vol. 64 Issue 5, p1062-1067. 6p. - Publication Year :
- 2004
-
Abstract
- To evaluate the oxidative status of the bladder 8 weeks after diabetes induction. Oxidative stress has recently been implicated in the pathogenesis of diabetes complications, but its role in diabetic cystopathy has not been studied.Sprague-Dawley rats were divided into three groups: control (n = 11), diuretic control (5% sucrose drink; n = 6), and streptozotocin-induced diabetic group (n = 14). Eight weeks later, the bladders were dissected. We measured the antioxidant scavenging enzymes (catalase and superoxide dismutase)-like activity and the levels of the thiobarbituric acid reactive substances, as a marker of lipid peroxidation. We also examined the levels of inducible nitric oxide synthase and apoptosis in the bladders.We found a statistically significant reduction in the catalase-like activity in the bladders from the diabetic group compared with the other groups (P = 0.017, diabetic versus control); the difference in the superoxide dismutase-like activity was not statistically significant among the groups. The thiobarbituric acid reactive substances levels were significantly greater in the diabetic compared with other groups (131.9 ± 47.5, 46.7 ± 17.9, and 60.9 ± 25.4 nmol/mg protein in the diabetic, control, and diuretic group, respectively, P = 0.006, diabetic versus control). Immunohistochemical and apoptosis studies showed a statistically significant increased number of inducible nitric oxide synthase-positive cells and apoptotic cells in the diabetic bladder smooth muscle cells (P <0.001).Our findings showed that oxidative stress occurred in the bladders of the STZ-diabetic rats and was not mediated by diuresis. The oxidative damage of the smooth muscle cells may be a contributory factor in diabetic cystopathy. [Copyright &y& Elsevier]
- Subjects :
- *OXIDATIVE stress
*BLADDER abnormalities
*DIABETES
*RATS
Subjects
Details
- Language :
- English
- ISSN :
- 00904295
- Volume :
- 64
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Urology
- Publication Type :
- Academic Journal
- Accession number :
- 14961283
- Full Text :
- https://doi.org/10.1016/j.urology.2004.06.021