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A supersensitive silicon nanowire array biosensor for quantitating tumor marker ctDNA.
- Source :
-
Biosensors & Bioelectronics . Jun2021, Vol. 181, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- Cancer has become one of the major diseases threatening human health and life. Circulating tumor DNA (ctDNA) testing, as a practical liquid biopsy technique, is a promising method for cancer diagnosis, targeted therapy and prognosis. Here, for the first time, a field effect transistor (FET) biosensor based on uniformly sized high-response silicon nanowire (SiNW) array was studied for real-time, label-free, super-sensitive detection of PIK3CA E542K ctDNA. High-response 120-SiNWs array was fabricated on a (111) silicon-on-insulator (SOI) by the complementary metal oxide semiconductor (CMOS)-compatible microfabrication technology. To detecting ctDNA, we modified the DNA probe on the SiNWs array through silanization. The experimental results demonstrated that the as-fabricated biosensor had significant superiority in ctDNA detection, which achieved ultralow detection limit of 10 aM and had a good linearity under the ctDNA concentration range from 0.1 fM to 100 pM. This biosensor can recognize complementary target ctDNA from one/two/full-base mismatched DNA with high selectivity. Furthermore, the fabricated SiNW-array FET biosensor successfully detected target ctDNA in human serum samples, indicating a good potential in clinical applications in the future. • A biosensor based on uniform dimensions high-response SiNW array was studied for PIK3CA E542K ctDNA detection. • 120 SiNWs with high consistency of dimensions make the signal superimpose, resulting in a higher signal-to-noise ratio. • Ultra-low detection limit (10 aM ctDNA) and a good linear relationship between 0.1 fM to 100 pM. • High specificity for the recognition of PIK3CA E542K ctDNA and excellent repeatability, reproducibility. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09565663
- Volume :
- 181
- Database :
- Academic Search Index
- Journal :
- Biosensors & Bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 149712791
- Full Text :
- https://doi.org/10.1016/j.bios.2021.113147