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Correlation between the synthesis of pullulan and melanin in Aureobasidium pullulans.
- Source :
-
International Journal of Biological Macromolecules . Apr2021, Vol. 177, p252-260. 9p. - Publication Year :
- 2021
-
Abstract
- The content of pullulan and melanin in 500 mutants of Aureobasidum pullulans obtained by ultraviolet mutagenesis were examined and statistically analyzed, and a strong positive correlation was found between them. The result was further confirmed by culturing wild type strain As3.3984 in different media. Then we constructed melanin-deletion mutant As- Δalb1 and pullulan-deletion mutant As- Δpul. As- Δalb1 was a melanin-free strain with the yield of pullulan decreased by 41.01%. The supplementation of melanin in the culture of As- Δalb1 increased the production of pullulan. As- Δpul synthesized neither pullulan nor melanin and recovered melanin synthesis by adding pullulan to the medium. The results suggested that high concentration- of pullulan induced morphological transformation and synthesis of melanin, and melanin promoted the synthesis of pullulan. The pullulan biosynthetic genes, upt , pgm , ugp , and pul , were down-regulated, while the negative regulatory gene of pullulan synthesis, creA , was up-regulated by melanin deficiency. • The synergistic correlation exists between the synthesis of pullulan and melanin. • High concentrations of pullulan induced the synthesis of melanin. • Melanin promotes the synthesis of pullulan. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01418130
- Volume :
- 177
- Database :
- Academic Search Index
- Journal :
- International Journal of Biological Macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 149780092
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2021.02.108