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A trans-complementation system for SARS-CoV-2 recapitulates authentic viral replication without virulence.
- Source :
-
Cell . Apr2021, Vol. 184 Issue 8, p2229-2229. 1p. - Publication Year :
- 2021
-
Abstract
- The biosafety level 3 (BSL-3) requirement to culture severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a bottleneck for research. Here, we report a trans -complementation system that produces single-round infectious SARS-CoV-2 that recapitulates authentic viral replication. We demonstrate that the single-round infectious SARS-CoV-2 can be used at BSL-2 laboratories for high-throughput neutralization and antiviral testing. The trans -complementation system consists of two components: a genomic viral RNA containing ORF3 and envelope gene deletions, as well as mutated transcriptional regulator sequences, and a producer cell line expressing the two deleted genes. Trans- complementation of the two components generates virions that can infect naive cells for only one round but does not produce wild-type SARS-CoV-2. Hamsters and K18-hACE2 transgenic mice inoculated with the complementation-derived virions exhibited no detectable disease, even after intracranial inoculation with the highest possible dose. Thus, the trans -complementation platform can be safely used at BSL-2 laboratories for research and countermeasure development. [Display omitted] • A trans -complementation system produces single-round infectious SARS-CoV-2 • Single-round infectious SARS-CoV-2 recapitulates authentic viral infection • Safety results support the trans -complementation system can be performed at BSL-2 • Trans -complementation assay can be used for high-throughput antiviral tests at BSL-2 Zhang et al. develop a trans- complementation system to produce single-round infectious SARS-CoV-2 that recapitulates authentic viral infection and replication. They then show the system can be safely used at biosafety level 2 for high-throughput antiviral testing. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SARS-CoV-2
*VIRAL replication
*VIRUS diseases
*COVID-19
Subjects
Details
- Language :
- English
- ISSN :
- 00928674
- Volume :
- 184
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Cell
- Publication Type :
- Academic Journal
- Accession number :
- 149781651
- Full Text :
- https://doi.org/10.1016/j.cell.2021.02.044