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Hydrazone derivative bearing coumarin for the relay detection of Cu2+ and H2S in an almost neat aqueous solution and bioimaging in lysosomes.
- Source :
-
Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy . Jul2021, Vol. 255, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
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Abstract
- [Display omitted] • The probe realized the relay detection of Cu2+ and H 2 S in an almost neat aqueous solution through fluorescence and UV–vis spectra. • The probe exhibits a high fluorescence quantum yield (ϕ = 0.48), a short response time (<30 s), and. • Low detection limits for Cu2+ (26 nM) and H 2 S (88.5 nM). • The probe has been applied for bioimaging of Cu2+ and H 2 S in lysosomes of living cells. A new morpholine functionalized coumarin-based fluorescent probe 1 was easily synthesized. The probe realized the sequentially detecting of Cu2+ and H 2 S in the HEPES buffer solution (20 mM, pH = 5.0). It made a turn-off fluorescence response to Cu2+ by using a complex formation with a 2:1 binding mode, and the resulting complex was able to detect H 2 S according to the displacement approach with a turn-on fluorescence response. The detecting limits of probe 1 for Cu2+ and 1 –Cu2+ system for H 2 S were calculated to be 26 nM and 88.5 nM, respectively. This "on-off-on" recognition process was demonstrated by ultraviolet–visible spectroscopy, fluorescence spectroscopy, using proton nuclear magnetic resonance studies, electrospray ionization mass spectroscopy, single crystal X-ray diffraction, and using density functional theory calculations. In addition, both cell imaging and co-staining experiments showed that the probe could be utilized to visually detect Cu2+ and H 2 S in lysosomes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13861425
- Volume :
- 255
- Database :
- Academic Search Index
- Journal :
- Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 149784018
- Full Text :
- https://doi.org/10.1016/j.saa.2021.119693