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TiO2-sensitized double-shell ZnCdS hollow nanospheres for photoelectrochemical immunoassay of carcinoembryonic antigen coupled with hybridization chain reaction-dependent Cu2+ quenching.
- Source :
-
Biosensors & Bioelectronics . Aug2021, Vol. 185, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
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Abstract
- A novel photoelectrochemical immunosensor was constructed to monitor carcinoembryonic antigen (CEA) based on hybridization chain reaction (HCR)-mediated in situ generation of copper nanoparticles (Cu NPs) and subsequent Cu2+-dependent quenching reaction, in which titanium dioxide nanoparticles-sensitized double-shell zinc cadmium sulfide hollow nanospheres (TiO 2 /DS-ZnCdS)-modified ITO electrode and anti-CEA antibody-modified 96-well plate served as biological recognition and signal detection platforms, respectively. The synergistic effect of TiO 2 NPs and DS-ZnCdS hollow nanospheres contributed to the improvement of photoelectric conversion efficiency, and HCR-mediated signal cascade benefited the enhancement of detection sensitivity. In the presence of CEA, biotin-labelled anti-CEA antibodies were immobilized onto anti-CEA antibody-modified 96-well plate, and triggered HCR process to form long double stranded DNA, which could adsorb a large number of Cu2+ ions and then in situ form Cu NPs on double stranded DNA template by a facile reduction reaction. After acid treatment, the dissolved Cu2+ ions could significantly reduce the photocurrent response due to the generation of Cu x S. Under optimal conditions, the immunosensor exhibited a desirable liner range of 1 pg mL−1 – 50 ng mL−1 and a low detection limit of 0.1 pg mL−1, as well as excellent selectivity and stability. Meanwhile, the recoveries of human serum sample analysis ranged from 96.8% to 103.6%, and the relative standard deviation was less than 7.40%, showing a good feasibility in early clinical diagnosis. • A novel photoelectrochemical immunosensor based on TiO 2 -sensitized DS-ZnCdS HNs was constructed for CEA detection. • HCR-mediated cascade process and Cu2+-based quenching reaction were integrated. • The photocurrent response was enhanced by TiO 2 /DS-ZnCdS heterostructure. • The excellent performances of designed immunosensor benefited to its potential application in early clinical diagnosis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09565663
- Volume :
- 185
- Database :
- Academic Search Index
- Journal :
- Biosensors & Bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 150295006
- Full Text :
- https://doi.org/10.1016/j.bios.2021.113251