帕瑞昔布钠对大鼠局灶脑缺血再灌注损伤的影响 .

Objective To investigate the effect and the mechanism of parecoxib pretreatment on focal cerebral ischemia-reperfusion injury in rats by measuring the content of TXA2，PGI2，IL-1β，TNF-α，PGE2 and TXA2/PGI2 in plasma. Methods Fourty-eight male SD rats were randomly divided into 4 groups （n＝12） : sham operation group （S）；model group （I/R）；parecoxib 5/10 mg/kg group （L， H） . Middle cerebral artery occlusion models were made by reforming Longa suture method in SD rats. Rats in L and H group were injected 5 mg/kg and 10 mg/kg parecoxib，respectively，through internal jugular vein 30 minutes before ischemia. Rats in S group and I/R group were given equal volume of normal saline. After ischemia for 90 mins and reperfusion for 24 hours， respectively， the neurologic deficit scores were measured， the pathological changes in CA1 region of hippocampus were detected by HE staining，radioimmunoassay technique was used to determine the content of TXA2，PGE2，TNF-α，IL-1β and TXA2/PGI2 in plasma. Results Pretreatment with parecoxib significantly decreased the content of TXA2，PGE2，TNF-α，IL-1β and TXA2/PGI2 in plasma， mitigated the injury in CA1 region of hippocampus and improved the nerve function of rats with focal cerebral ischemia-reperfusion injury. Conclusion Parecoxib can protect the brain from focal cerebral I/R injury by reducing the content of TXA2， PGE2， TNF-α， IL-1β and the ratio of TXA2/PGI2 in plasma of rats. [ABSTRACT FROM AUTHOR]