慈竹扦插苗移栽后不同时期的生理适应性.

In order to investigate the environmental adaptation of Neosinocalamus affinis cuttings after transplantation, we used paraffin section technique to observe the anatomical structure of leaves, and the phenol-sulfuric acid method to determine the photosynthetic characteristics. The results revealed that with prolonged post-transplantation duration, the stomata of the cutting seedling leaves became smaller, although there were increases in stomatal density, leaf area and the thickness, cuticles, and upper and lower epidermis cells. Moreover, we observed an increase in the area of bulliform cells, which penetrated more deeply into the mesophyll, but the area of spindle cells was reduced. Furthermore, the net photosynthetic rate of leaves increased while the transpiration rate decreased. The contents of water and soluble sugars in leaves decreased, whereas the dry fresh weight ratio, the starch content, and the number of starch granules in leaves increased. We also noted a gradual accumulation of photosynthetic products in the leaves of cuttings with time following transplantation, which contributed to enhancing their environmental adaptation. Notably, the leaf structures and photosynthetic indices of cuttings observed at three months post-transplantation were similar to those characterizing 3-year-old seedlings. Therefore, reasonable protective measures should be taken during the first three months after transplantation using cuttings to prevent excessive water loss and enhance the survival of cuttings [ABSTRACT FROM AUTHOR]