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miR-760 mediated the proliferation and metastasis of hepatocellular carcinoma cells by regulating HMGA2.
- Source :
-
Pathology - Research & Practice . Jun2021, Vol. 222, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- The purpose of our study was to investigate the roles of miR-760 and its potential mechanisms in HCC. The functions of miR-760 were identified and measured by MTT, colony formation, transwell, and flow cytometry assays. Luciferase assay was applied to verify the direct binding of miR-760 on HMGA2 3′untranslated region (3′UTR). Then, in vitro experiment was used to investigate the biological effects of miR-760 and HMGA2. Luciferase and ChIP assays were used to detect the validity of SP1 binding sites on the miR-760 promoter. We demonstrated that miR-760 overexpression suppressed cell proliferation, migration, and invasion in HCC. Besides, HMGA2 was demonstrated as a direct target gene of miR-760. Furthermore, we found that methylation may result in the downregulation of miR-760, and SP1 could inhibit the transcription of miR-760. Our study demonstrated that SP1/miR-760/HMGA2 may serve as a molecular regulatory axis for HCC treatment. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03440338
- Volume :
- 222
- Database :
- Academic Search Index
- Journal :
- Pathology - Research & Practice
- Publication Type :
- Academic Journal
- Accession number :
- 150575614
- Full Text :
- https://doi.org/10.1016/j.prp.2021.153420