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HSF1 promotes endometriosis development and glycolysis by up-regulating PFKFB3 expression.
- Source :
-
Reproductive Biology & Endocrinology . 6/9/2021, Vol. 19 Issue 1, p1-10. 10p. - Publication Year :
- 2021
-
Abstract
- Background: Endometriosis is a chronic hormonal inflammatory disease characterized by the presence of endometrial tissue outside the uterus. Endometriosis often causes infertility, which brings physical and mental pain to patients and their families. Methods: We examined the functions of heat shock factor 1 (HSF1) in endometriosis development through cell count assay, cell-scratch assay and clone formation experiments. We used quantitative real-time PCR (qRT-PCR) and Western blot (WB) to detect HSF1 expression. Glucose and lactate levels were determined using a glucose (GO) assay kit and a lactate assay kit. Furthermore, we used a HSF1 inhibitor-KRIBB11 to establish a mouse model of endometriosis. Results: Our data demonstrated that HSF1 promoted endometriosis development. Interestingly, HSF1 enhanced glycolysis via up-regulating PFKFB3 expression in endometriosis cells, which was a key glycolysis enzyme. Consistently, the HSF1 inhibitor KRIBB11 could abrogate endometriosis progression in vivo and in vitro. Conclusions: Findings indicate that HSF1 plays an important role in endometriosis development, which might become a new target for the treatment of endometriosis. Electronic supplementary material: Supplementary data are available. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ENDOMETRIOSIS
*HEAT shock factors
*GLYCOLYSIS
*LABORATORY mice
*ENTHALPY
Subjects
Details
- Language :
- English
- ISSN :
- 14777827
- Volume :
- 19
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Reproductive Biology & Endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 150793791
- Full Text :
- https://doi.org/10.1186/s12958-021-00770-9