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Characterization of Litopenaeus vannamei secreted protein acidic and rich in cysteine -like in WSSV infection.

Authors :
Zou, Rui-Feng
Ren, Xing-Chao
Liu, Qing-Hui
Source :
Journal of Invertebrate Pathology. Jul2021, Vol. 183, pN.PAG-N.PAG. 1p.
Publication Year :
2021

Abstract

[Display omitted] • LvSPARC-L contained an open reading frame of 1002 bp, encoding 333 amino acids. • LvSPARC-L was mainly expressed in hemocyte, stomach and pleoplod. • LvSPARC-L was significantly up-regulated by WSSV challenge. • IE1 - VP28 were significantly increased in LvSPARC-L -silenced shrimp. • LvP53 and LvCaspase3 was extremely decreased in LvSPARC-L -silenced shrimp. Secreted protein acidic and rich in cysteine (SPARC) is an extracellular and non-structural glycoprotein. In shrimp, a significant function of SPARC in WSSV infection remains unclear. In this study, the full-length cDNA sequence of a secreted protein acidic and rich in cysteine -like was cloned from shrimp Litopenaeus vannamei (named as LvSPARC-L). LvSPARC-L contained an open reading frame of 1002 bp, encoding 333 amino acids. Bioinformatics analysis showed that LvSPARC-L contained a SPARC Ca2+-binding region in the C-terminus, a Kazal-type serine protease inhibitor domain and a BUD22 domain. Tissue distribution assay indicated that LvSPARC-L generally expressed in all tissues selected with a higher expression in hemocyte, stomach and pleoplod. In hepatopancreas and intestine, the relative expression of LvSPARC-L was significantly up-regulated following the WSSV challenge. Besides, the relative expression of viral immediately early gene IE1 and a late gene VP28 was significantly increased in the LvSPARCL -silenced shrimp. Furthermore, the relative expression of LvP53 and LvCaspase3 was extremely decreased in the stomach of ds LvSPARC-L treated shrimp, while that of LvP38 was not affected significantly. All data together suggest that LvSPARC-L might play an antiviral role by regulating apoptosis. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00222011
Volume :
183
Database :
Academic Search Index
Journal :
Journal of Invertebrate Pathology
Publication Type :
Academic Journal
Accession number :
150851517
Full Text :
https://doi.org/10.1016/j.jip.2021.107593