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A new fluorescent probe for neutral to alkaline pH and imaging application in live cells.
- Source :
-
Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy . Nov2021, Vol. 261, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- [Display omitted] • A new pH sensitive fluorescent probe termed NAP-MDA is developed. • NAP-MDA possesses an excellent linear dependence with neutral to alkaline pH. • NAP-MDA is photostable and solely sensitive to pH. • NAP-MDA is successfully utilized to visualize the intracellular pH. A new pH-sensitive fluorescent probe NAP-MDA was designed and synthesized. NAP-MDA consists of 1,8-naphthalimide as fluorophore, morpholine and N,N-dimethylethylenediamine as pH-responsive groups. Due to the photoinduced electron transfer (PET) mechanism, the fluorescence of 1, 8-naphthalimide was thoroughly quenched under alkaline condition (pH > 10.0), however, NAP-MDA displayed increasing fluorescence as the rise of acidity. Notably, NAP-MDA possessed an excellent linear dependence with neutral to alkaline pH (7.2–9.4), with a pKa of 8.38. NAP-MDA had good photostability and reversibility. Meanwhile, the probe was selective to pH without interference from common reactive species, temperature and viscosity. Fluorescent testing strips were fabricated with NAP-MDA and were successfully utilized to visualize the different pH with a handhold UV lamp. Confocal fluorescence imaging in live cells demonstrated that NAP-MDA mainly fluoresced in lysosomes, and could be applied for quantification of the pH within live cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13861425
- Volume :
- 261
- Database :
- Academic Search Index
- Journal :
- Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 151124111
- Full Text :
- https://doi.org/10.1016/j.saa.2021.120031