Back to Search
Start Over
Immune adjuvant effects of interferon-gamma (IFN-γ) of flounder (Paralichthys olivaceus) against Edwardsiella tarda.
- Source :
-
Developmental & Comparative Immunology . Oct2021, Vol. 123, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- IFN-γ plays a key role in T-cell activation and the establishment of the adaptive immune response, which has a potential as a cytokine adjuvant in the context of vaccination. In this study, we evaluated the immune adjuvant effects of two forms of flounder (Paralichthys olivaceus) IFN-γ, including pcDNA3.1-IFN-γ (pcIFN-γ) and recombinant IFN-γ (rIFN-γ), and comparatively analyzed the immune responses of flounder to E. tarda subunit vaccine rOmpV. The results showed that vaccination with rOmpV plus pcIFN-γ or rIFN-γ produced a relative percent survival of 57% and 71%, respectively, which were significantly higher than that of the control groups, rOmpV plus pcN3 (36%) or rHis (40%). Compared with the two control groups, vaccination with rOmpV plus pcIFN-γ or rIFN-γ could induce significantly higher levels of specific serum antibodies and sIg + lymphocytes in peripheral blood, spleen and head kidney, and significantly higher upregulated expressions of CD4-1, CD8α, IgM, MHC Ⅰα, MHC Ⅱα, IL-1β and TNF-α were also detected in rOmpV plus pcIFN-γ or rIFN-γ vaccinated fish. In addition, compared with pcIFN-γ, rOmpV co-vaccination with rIFN-γ elicited higher levels of sIg + lymphocytes, specific serum antibodies and several immune-related genes expressions in vaccinated flounder. These results demonstrated that rOmpV co-vaccination with rIFN-γ or pcIFN-γ could both boost the immune responses and evoke highly protective effects against E. tarda , indicating that flounder IFN-γ is a promising adjuvant candidate for fish vaccination via an injection administering route. • Flounder IFN-γ displayed strong adjuvant stimulatory activity. • The rIFN-γ and pcIFN-γ both can enhance the immune protective efficacy of subunit vaccines rOmpV. • Recombinant IFN-γ combined with rOmpV can elicit stronger immune response and confer higher RPS. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0145305X
- Volume :
- 123
- Database :
- Academic Search Index
- Journal :
- Developmental & Comparative Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 151172738
- Full Text :
- https://doi.org/10.1016/j.dci.2021.104159