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Potential effect of hyaluronic acid and triamcinolone acetate, alone or combined, on chondrogenic differentiation of mesenchymal stem cells.
- Source :
-
Revista Colombiana de Ciencias Pecuarias . Jul-Sep2021, Vol. 34 Issue 3, p212-223. 12p. - Publication Year :
- 2021
-
Abstract
- Osteoarthritis is a complex degenerative disease with several factors contributing to joint damage. Objective: To compare the potential effect of hyaluronic acid (HA) and triamcinolone acetonide (TA), alone or combined, on the in vitro chondrogenic differentiation process of mesenchymal stem cells (MSCs). Methods: MSCs were divided into four groups: Control, HA, TA, and HA/TA combined. Each treatment group was cultured for 14 days in chondrogenic differentiation medium. The chondrogenic differentiation potential was assessed by histology and immunohistochemistry. Results: The HA and HA/TA-treated MSCs presented histological characteristics similar to native chondrocytes. The extracellular matrix (ECM) of TA-treated MSCs was compact and organized. Glycosaminoglycan staining was intense in Control, moderate in TA, slight in HA/TA, and undetectable in HA. Type II collagen immunoreactivity was high in the TA-treated ECM and MSCs. Conclusions: Histological analysis shows that HA influences morphological development similar to chondrocytes of the MSCs, but with low expression of specific cartilage molecules. The TA promotes formation of a compact and organized ECM. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01200690
- Volume :
- 34
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Revista Colombiana de Ciencias Pecuarias
- Publication Type :
- Academic Journal
- Accession number :
- 151184782
- Full Text :
- https://doi.org/10.17533/udea.rccp.v34n3a06