Back to Search
Start Over
6-Shogaol from ginger shows anti-tumor effect in cervical carcinoma via PI3K/Akt/mTOR pathway.
- Source :
-
European Journal of Nutrition . Aug2021, Vol. 60 Issue 5, p2781-2793. 13p. 1 Color Photograph, 6 Graphs. - Publication Year :
- 2021
-
Abstract
- Purpose: 6-Shogaol, an active phenolic compound from ginger (Zingiber officinale), can inhibit the growth of a variety of human cancer cells. Nevertheless, its underlying molecular mechanisms in cervical cancer remain unclear. In this study, we systematically examine the inhibitory effect of 6-shogaol on cervical cancer in vitro and in vivo. Methods: Cell proliferation was assessed by CCK8 assay and colony formation assay in HeLa and SiHa cells. We analyzed cell cycle and apoptosis through flow cytometry. GFP-LC3 puncta and transmission electron microscopy were used to observe autophagic bodies. Wound-healing assay and transwell assay were used for evaluating the migration of cells. Western blot was applied to detect protein expression levels. Results: 6-Shogaol could suppress cell proliferation and migration, cause cell cycle arrest in the G2/M phase in HeLa and SiHa cells. Moreover, 6-shogaol triggered the apoptosis process through the mitochondrial pathway by downregulating the expression levels of p-PI3K, p-Akt and p-mTOR. Further research indicated that the induction of apoptosis by 6-shogaol was remarkably decreased after the treatment of ROS scavenger and PI3K agonist. Additionally, 6-shogaol increased the number of LC3-positive puncta and autophagic bodies per cell in both HeLa and SiHa cells. Pretreatment of cells with Bafilomycin A1, an autophagy inhibitor, accelerated 6-shogaol mediated cell apoptosis, suggesting that induction of autophagy by 6-shogaol is suppressive to apoptosis. Furthermore, in vivo data revealed that 6-shogaol significantly inhibited tumor growth and cell proliferation in tumor tissues. Conclusion: These findings suggested that 6-shogaol could be developed as a functional food ingredient, which is potentially used as therapeutic agents for patients with cervical cancer. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BIOLOGICAL models
*IN vitro studies
*FLOW cytometry
*WOUND healing
*GINGER
*PHENOLS
*IN vivo studies
*PHOSPHOTRANSFERASES
*ANIMAL experimentation
*COLONY-forming units assay
*AUTOPHAGY
*WESTERN immunoblotting
*ANTINEOPLASTIC agents
*SIGNAL peptides
*APOPTOSIS
*RATS
*CELL cycle
*ELECTRON microscopy
*GENE expression
*CELL proliferation
*PLANT extracts
*MOLECULAR structure
*BIOLOGICAL assay
*PHARMACODYNAMICS
CERVIX uteri tumors
Subjects
Details
- Language :
- English
- ISSN :
- 14366207
- Volume :
- 60
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- European Journal of Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 151386821
- Full Text :
- https://doi.org/10.1007/s00394-020-02440-9