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Peperomin E Induces Apoptosis and Cytoprotective Autophagy in Human Prostate Cancer DU145 Cells In Vitro and In Vivo.
- Source :
-
Planta Medica . 2021, Vol. 87 Issue 8, p620-630. 11p. - Publication Year :
- 2021
-
Abstract
- Peperomin E was first isolated from Peperomia dindygulensis , an anticarcinogenic herb, and exhibited anticancer activity in many cancer cell lines. To date, it is unknown whether peperomin E has an effect on human prostate cancer DU145 cells in vitro and in vivo. In this study, we used MTT to assess the proliferation inhibition activity of peperomin E in DU145 cells in vitro and observed the cell morphological changes by a phase contrast microscope. A DU145 cell xenograft tumor mouse model was used to evaluate the efficacy of peperomin E in vivo. Apoptosis rates were measured by flow cytometry, and protein expression levels were analyzed by western blot. The results showed that peperomin E significantly inhibited the proliferation of DU145 cells in vitro and reduced the weight and volume of tumors in vivo. Peperomin E also significantly induced the apoptosis and autophagic response of DU145 cells. The autophagic inhibitors LY294002 and chloroquine enhanced peperomin E-mediated inhibition of DU145 cell proliferation and induction of DU145 cell apoptosis. The results also showed that the Akt/mTOR pathway participated in peperomin E-induced autophagy in DU145 cells. In summary, our finding showed that peperomin E had an effect on DU145 cells in vitro and in a nude mouse DU145 cell xenograft model in vivo , demonstrated that peperomin E could significantly induce apoptosis and the autophagic response in DU145 cells and that autophagy played a cytoprotective role in peperomin E-treated DU145 cells. These results suggest that the combination of peperomin E treatment and autophagic inhibition has potential for the treatment of prostate cancer. [ABSTRACT FROM AUTHOR]
- Subjects :
- *IN vitro studies
*BIOLOGICAL models
*MEDICINAL plants
*IN vivo studies
*AUTOPHAGY
*ANIMAL experimentation
*ANTINEOPLASTIC agents
*APOPTOSIS
*SIGNAL peptides
*PHYTOCHEMICALS
*CELLULAR signal transduction
*CELL proliferation
*CELL lines
*MOLECULAR structure
*PROSTATE tumors
*MICE
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 00320943
- Volume :
- 87
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Planta Medica
- Publication Type :
- Periodical
- Accession number :
- 151403500
- Full Text :
- https://doi.org/10.1055/a-1348-1634