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Specific determination of HBV using a viral aptamer molecular imprinting polymer sensor based on ratiometric metal organic framework.
- Source :
-
Microchimica Acta . Jul2021, Vol. 188 Issue 7, p1-9. 9p. - Publication Year :
- 2021
-
Abstract
- An approach is reported based on the combination of aptamer and metal organic frameworks (MOF) to prepare a molecularly imprinted sensor that recognizes viruses with high specificity and sensitivity. Using MIL-101-NH2 as a polymer carrier, viral aptamers were introduced into the carrier surface through an amide reaction to specifically identify the target, and surface imprinting is carried out through tetraethyl silicate (TEOS) self-polymerization. The MIL-101-NH2 is also used as the reference fluorescence signal (λex/λem = 290/460 nm) and rhodamine B as the change signal (λex/λem = 550/570 nm). The ratiometric fluorescence detection and dual recognition strategy not only reduce environmental interference but also greatly improve the sensor’s anti-interference ability, the obtained imprinting factor was 5.72, and the detection limit as low as 1.8 pmol L−1. Therefore, the molecular imprinting sensor designed realizes the specific and highly sensitive identification of viruses, which provides theoretical support for the application of molecular imprinting technology in clinical diagnosis of viruses. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 188
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 151449400
- Full Text :
- https://doi.org/10.1007/s00604-021-04858-2