Seven undescribed steroids from the leaves of Datura metel L.

[Display omitted] • Seven undescribed steroids were isolated from the leaves of Datura metel L. • Compounds 1 and 4 contain as a unique 1,10- seco -withanolide moiety. • Daturmetelide X (1) exhibited potential anti-inflammatory activity. Extraction of Datura metel L. leaves with ethanol as a solvent gave a group of steroids, including two unique 1,10- seco -withanolides (1 , 4), an unusual nitrogen-containing withanolides (2), one undescribed saponin (3), two withanolides with a carbohydrate (5 , 6), and one C 21 steroid (7). These compounds' structures were identified based on HR-ESI-MS and 1H, 13C NMR data analyses, also compared with data from the document. Some compounds showed moderate inhibition on NO production in lipopolysaccharide-stimulated RAW 264.7 cells. [ABSTRACT FROM AUTHOR]