Amorphous carbon nitride microband integrated in a microfluidic device for DNA biosensors applications.

• Integration of amorphous carbon nitride (a-CNx) was reported in microfluidic. • Hydrodynamic voltammetry and EIS were used for characterization under flow. • The developed electrochemical biosensor is robust, label free, and stable. • We evidenced how an activation procedure leads more DNA probes collection. • The use of a-CNx microelectrodes for DNA detection is evidenced. This study presents the use of new kind of carbon electrode materials as ultramicroelectrodes (UMEs) in the field of electrochemical DNA biosensors which has already been proven to be effective in protocols to DNA sequences hybridization. In contrast to other carbon materials such as diamond like carbon, that are difficult to integrate in microfluidic devices due to their high temperature deposition, amorphous carbon nitride (a-CNx) is easily synthesized at room temperature on various materials using sputtering techniques. Here, we report a-CNx use as microband electrodes in Glass/PDMS microfluidic devices. a-CNx electrodes were activated and then biofuntionalized by covalent grafting of a DNA probe as self-assembled monolayer (SAM) with a view to future development of a detection platform targeting circulating DNA or RNA sequences in microfluidic channels. [ABSTRACT FROM AUTHOR]