Germline soma communication mediated by gap junction proteins regulates epithelial morphogenesis.

Gap junction (GJ) proteins, the primary constituents of GJ channels, are conserved determinants of patterning. Canonically, a GJ channel, made up of two hemi-channels contributed by the neighboring cells, facilitates transport of metabolites/ions. Here we demonstrate the involvement of GJ proteins during cuboidal to squamous epithelial transition displayed by the anterior follicle cells (AFCs) from Drosophila ovaries. Somatically derived AFCs stretch and flatten when the adjacent germline cells start increasing in size. GJ proteins, Innexin2 (Inx2) and Innexin4 (Inx4), functioning in the AFCs and germline respectively, promote the shape transformation by modulating calcium levels in the AFCs. Our observations suggest that alterations in calcium flux potentiate STAT activity to influence actomyosin-based cytoskeleton, possibly resulting in disassembly of adherens junctions. Our data have uncovered sequential molecular events underlying the cuboidal to squamous shape transition and offer unique insight into how GJ proteins expressed in the neighboring cells contribute to morphogenetic processes. Author summary: Shape transitions between different subtypes of epithelial cells i.e., cuboidal, squamous and columnar are ubiquitous and are essential during organogenesis across animal kingdom. We demonstrate that heteromeric combination of gap junction proteins, Drosophila Innexin2 and Drosophila Innexin 4 (also known as Zero population growth or Zpg), expressed in the soma and germline of fly egg respectively, mediates the shape transition of cuboidal follicle cells to squamous fate. Interestingly, the two gap junction proteins likely participate as constituents of a calcium channel. Further, we show that somatic follicle cells and germline nurse cells communicate through calcium fluxes that activates STAT in the follicle cells. Activated STAT modulates the levels/ activity of junctional complexes thus aiding shape transition of cuboidal cells to squamous fate. These findings provide novel insights into how communication between different cell types with distinct origins achieve shape transitions essential for proper organ assemblies. [ABSTRACT FROM AUTHOR]